Thermo Fisher Scientific Coilin Polyclonal Antibody
Thermo Fisher Scientific의 Coilin Polyclonal Antibody는 인간 Coilin 단백질을 인식하는 토끼 유래 IgG 항체로, Western blot, IHC, ICC, IP 등 다양한 응용에 적합합니다. 고순도 항원 친화 크로마토그래피 정제 제품으로 안정적인 액상 형태로 제공되며 연구용으로 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View publication |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 1–237 of Human Coilin (UniProt ID: P38432-1, NCBI Node ID: 9606) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.66 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2547007 |
Product Specific Information
- Recommended positive controls: HepG2, HepG2 nuclear extract, 293T nuclear extract
- Predicted reactivity: Rhesus Monkey (95%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The Coilin protein is an integral component of Cajal bodies (also called coiled bodies), which are nuclear suborganelles involved in post-transcriptional modification of small nuclear and small nucleolar RNAs.
The N-terminus of Coilin directs self-oligomerization, while the C-terminus influences the number of nuclear bodies assembled per cell.
Differential methylation and phosphorylation of Coilin likely affect its localization and assembly within nuclear bodies.
This gene has pseudogenes on chromosome 4 and chromosome 14.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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