Thermo Fisher Scientific P-Selectin Monoclonal Antibody (Psel.KO.2.7)

Applications

Immunohistochemistry (Frozen) (IHC (F))

• Tested Dilution: Assay-dependent

Flow Cytometry (Flow)

• Tested Dilution: 1:25–1:50

Immunoprecipitation (IP)

• Tested Dilution: Assay-dependent

Product Specifications

Additional Formats

• PE Conjugate
• Request custom conjugation

Product Specific Information

For FACS analysis, use 10 µL of the suggested working dilution to label 1×10^6 cells in 100 µL.
Mouse anti-human CD62P, clone Psel.KO.2.7, recognizes human P-Selectin.

Target Information

P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type I transmembrane glycoprotein that regulates cell rolling. It is stored in the Weibel-Palade bodies of endothelial cells and α-granules of platelets, and can be rapidly translocated to the cell surface upon stimulation by thrombin, histamine, complement 5a, Ca²⁺ ionophores, or adenosine diphosphate.
During platelet activation and degranulation, P-selectin mediates interactions between activated endothelial cells or platelets and leukocytes. It plays a key role in leukocyte-endothelial adhesion and is a calcium-dependent receptor binding sialylated Lewis carbohydrate antigens on neutrophils and monocytes.
P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis, and tissue destruction. Clinically, it is used to distinguish heparin-induced thrombocytopenia with and without thrombosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.