Thermo Fisher Scientific CyQUANT MTT Cell Viability Assay

Invitrogen™ CyQUANT™ MTT Cell Viability Assay

The CyQUANT™ MTT Cell Viability Assay utilizes the well-established and widely used MTT reagent to determine mammalian cell viability. The redox potential in active mammalian cells reduces MTT to a strongly pigmented formazan product. After solubilization, the absorbance of the formazan can be measured with a microplate absorbance reader.

This assay kit provides all reagents necessary for the detection of mammalian cell viability and includes sufficient materials for approximately 1,000 assays.

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Features

• Complete and optimized kit for colorimetric detection of viable mammalian cells
• Utilizes the well-established MTT reagent as a reporter of cellular redox potential
• Configured to perform 1,000 tests using a 96-well microplate

Description

Measuring changes in cell viability is a fundamental method for assessing cell health, determining genotoxicity, and evaluating anti-cancer drugs. Colorimetric indicators offer a rapid and cost-effective method for determining changes in mammalian cell viability, and the MTT assay is one of the most established and popular options.

The redox potential in viable mammalian cells converts the water-soluble MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) into an insoluble formazan product. After solubilization of the formazan with the included SDS (sodium dodecyl sulfate) reagent, the concentration of the colorimetric probe is determined by measuring optical density at 570 nm.

The CyQUANT MTT Cell Viability Assay provides a simple and reliable method for determining mammalian cell viability using standard microplate absorbance readers. Simply prepare the MTT reagent, add it to the cells, solubilize the resulting formazan, and measure the optical density using a microplate reader.

Specifications