Thermo Fisher Scientific GLUT-1 Monoclonal Antibody (GLUT1, 2475)
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GLUT1 단백질을 인식하는 Mouse monoclonal 항체로 Western blot, IHC, ICC, Flow Cytometry 등에 사용 가능. Human 시료에 반응하며, Protein A/G 정제 및 PBS buffer에 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GLUT1, 2475 |
| Immunogen | Recombinant fragment of human GLUT1 protein (around aa 203–305) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Facilitative glucose transporter (GLUT1).
This isoform may be responsible for constitutive or basal glucose uptake.
Has a broad substrate specificity and can transport a wide range of aldoses, including both pentoses and hexoses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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