Thermo Scientific™

Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3`-(2-pyridyldithio)propionamido)hexanoate)

Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form자세히 알아보기

Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteines.

Features of Sulfo-LC-SPDP:

• Reactive groups: NHS ester and pyridyldithiol
• Reactive towards: amino and sulfhydryl groups
• Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
• Water soluble, sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
• Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
• Spacer arm is also easily cleaved using reducing SDS-PAGE sample loading buffer
• Cleavable crosslinker allows separation of crosslinked products
• Sulfo-LC-SPDP crosslinker is not membrane permeable, allowing cell surface labeling
• Compare to other varieties of SPDP-type reagents, including pegylated forms

Sulfo-LC-SPDP is Sulfosuccinimidyl 6-(3`-[2-pyridyldithio]-propionamido)hexanoate, a heterobifunctional, thiol-cleavable and membrane impermeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide (NHS) ester that will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in the pyridyl disulfide group that will react with sulfhydryls to form a reversible disulfide bond.

Applications:
• Reversible crosslinking of proteins
• Adding sulfhydryl groups to proteins by attachment to lysine residues
• Protein crosslinking to create antibody-enzyme and other bioconjugates via a two-step reaction
• Attaching haptens to carrier proteins for antibody production
• Preparing immunotoxin conjugates

Specifications:
We manufacture Sulfo-LC-SPDP to the highest specifications to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of Sulfo-LC-SPDP is tested to meet the following minimum specifications.

• Identity: IR scan shows only peaks characteristic of the structure and functional groups of Sulfo-LC-SPDP crosslinker
• Solubility: ≥ 95 mg/mL in DI water
• Purity: ≥ 90% by quantitative NMR (the highest standard for crosslinker purity)

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
Sulfo-LC-SPDP (sulfosuccinimidyl 6-(3`-(2-pyridyldithio)propionamido)hexanoate)
SPDP (succinimidyl 3-(2-pyridyldithio)propionate)

사양

화학물질 반응성Amine-Sulfhydryl

CleavableBy Thiols

설명Sulfo-LC-SPDP

분자량527.57

페길레이티드No

스페이서 암 길이15.7 Å

세포 투과성No

배송 조건Ambient

제품라인Pierce™

라벨링 방법Chemical Labeling

교차결합제 유형Heterobifunctional

반응성 부분Pyridyldisulfide, Sulfo-NHS Ester

스페이서Medium (10 to 30 Å)

형태Powder

수량50 mg

용해도Water

형식Standard

수용성Yes

제품 유형Crosslinker

Unit SizeEach