Thermo Fisher Scientific ROBO1 Polyclonal Antibody
ROBO1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체입니다. Western blot, ICC/IF, ELISA에 사용 가능하며, 인간, 마우스, 랫트 시료에 반응합니다. 액상 형태로 제공되며, -20°C에서 보관합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1452–1651 of human ROBO1 (NP_002932.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.47 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913916 |
Product Specific Information
Positive test controls include: 293T, U-251MG, HeLa, Mouse brain, Rat brain.
The target is usually found in the following locations: Cell membrane, Cell projection, Single-pass type I membrane protein, axon.
Immunogen sequence:
AAVMQKTRPA KKLKHQPGHL RRETYTDDLP PPPVPPPAIK SPTAQSKTQL EVRPVVVPKL PSMDARTDRS SDRKGSSYKG REVLDGRQVV DMRTNPGDPR EAQEQQNDGK GRGNKAAKRD LPPAKTHLIQ EDILPYCRPT FPTSNNPRDP SSSSSMSSRG SGSRQREQAN VGRRNIAEMQ VLGGYERGED NNEELEETES
Target Information
Specialized cells at the midline, which separates the left and right halves of the CNS, have roles in directing growth cone behavior. In vertebrates, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion.
Robo1 and Robo2 are human homologs of the Drosophila protein Roundabout. Robo1 is homologous to the C. elegans gene sax3, and Robo2 to the zebrafish gene astray.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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