Thermo Fisher Scientific Casc5 Polyclonal Antibody
Rabbit polyclonal antibody against human Casc5, validated for ICC/IF. Recognizes recombinant human Casc5 protein. Suitable for mitosis and kinetochore research. Supplied as liquid at 0.2 mg/mL in PBS with 40% glycerol. For research use only.
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Applications
- Immunocytochemistry (ICC/IF): 0.25–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human Casc5. Recombinant protein control fragment (Product #RP-106240) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2664140 |
Product Specific Information
Immunogen sequence:
DVTKQVIQTHV NAGEAPDPVI TSNVPCFHSI KPNLNNLNGK TGEFLAFQTV HLPPLPEQLL ELGNKAHNDM HIVQATEIHN INIISSNAKD SRDEENKKSH NGAETTSLPP KTVFKDKVRR CSLGIFLPRL PNKRNCSVTG IDDLEQ
Highest antigen sequence identity to the following orthologs:
- Mouse: 60%
- Rat: 60%
Target Information
Casc5 performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. It is required for attachment of the kinetochores to the spindle microtubules and directly links BUB1 and BUB1B to kinetochores. Casc5 is part of the MIS12 complex, fundamental for kinetochore formation and proper chromosome segregation during mitosis. It acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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