Thermo Fisher Scientific GAPDH Loading Control Polyclonal Antibody, Biotin
GAPDH 단백질 검출용 Western blot 로딩 컨트롤 항체. 인간, 마우스, 랫트 반응성. Biotin 결합된 염소 폴리클로날 항체로 높은 특이성 및 안정성 제공. 연구용으로만 사용 가능.
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Applications
- Western Blot (WB): 0.3–1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence C-HQVVSSDFNSDT (aa 279–290) |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932774 |
Product Specific Information
This antibody is tested in Peptide ELISA with an antibody detection limit dilution of 1:64,000.
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis and exists as a tetramer of identical 37-kDa subunits. It catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond glycolysis, GAPDH is ubiquitously expressed and participates in DNA replication, repair, RNA export, membrane fusion, and microtubule bundling. It plays roles in apoptosis, neurodegenerative diseases, and cancer-related processes. GAPDH interacts with proteins such as amyloid precursor protein and Huntingtin, and associates with actin and tubulin.
Due to its high expression in most tissues, GAPDH serves as a reliable protein loading control in Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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