Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), NovaFluor Yellow 590, eBioscience

Applications

• Flow Cytometry (Flow): Tested at ≤ 0.5 µg/test

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 488, 532, 561, 660, 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet series, eFluor series, FITC, PE, PE-Cyanine, PerCP, Super Bright, NovaFluor Blue/Red/Violet/Yellow series, and custom conjugation options.

Product Specific Information

Description:
The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as a heterodimer (CD8αβ) or homodimer (CD8αα). CD8 binds MHC class I and associates with p56lck to mediate T cell development and activation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis of mouse splenocytes.
Applications Tested: Optimal staining at ≤ 0.5 µg/test (100 µL final volume). Adjust cell number empirically (10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) with all NovaFluor conjugates. Add 5 µL per sample to antibody cocktails before combining with cells.
• For single-color controls: add 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology, based on engineered nucleic acid dye structures designed for optimized fluorescence with minimal spillover and spread.

Spectral Properties:
Excitation: 552 nm
Emission: 592 nm
Laser: 561 nm (Yellow)

Note:
Internal testing shows NovaFluor Yellow 590 may non-specifically stain B cells in SJL mice but not in BALB/c or C57BL/6 strains.

Target Information

CD8 (Cluster of Differentiation 8):
A type I transmembrane glycoprotein of the immunoglobulin superfamily involved in T cell signal transduction, differentiation, and activation.

• Expressed mainly on cytotoxic T cells as CD8αβ heterodimers.
• Also found as CD8αα homodimers on subsets of T cells, NK cells, monocytes, and dendritic cells.
• Functions as a co-receptor for MHC-I molecules, aiding in antigen recognition and CTL activation.
• Activation triggers Lck recruitment, cytokine production, and cytotoxic responses.
• Differentiation of naïve CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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