Thermo Fisher Scientific MAPKAPK3 Monoclonal Antibody (2B5)
MAPKAPK3 단백질을 표적하는 Thermo Fisher Scientific의 단클론 항체로, 인간 및 생쥐 반응성을 보입니다. Western blot, IHC, ELISA, PLA 등 다양한 응용에 적합하며, 고순도 친화 크로마토그래피로 정제된 액상 형태입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3 µg/mL |
| ELISA | 0.03 ng/mL |
| in situ PLA (PLA) | 1:1200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B5 |
| Immunogen | MAPKAPK3 (AAH01662, 272 a.a. approximately 382 a.a) partial recombinant protein with GST tag. MW of GST tag alone: 26 kDa. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein:
EVSEDAKQLI RLLLKTDPTE RLTITQFMNH PWINQSMVVP QTPLHTARVL QEDKDHWDEV KEEMTSALAT MRVDYDQVKI KDLKTSNNRL LNKRRKKQAG SSSASQGCNN QTarget Information
MAPKAPK3 functions as an integrative element of signaling in both mitogen and stress responses. It acts as a mitogen-activated protein kinase (MAP kinase)-activated protein kinase. MAP kinases (ERKs) serve as integration points for multiple biochemical signals.
MAPKAPK3 is activated by growth inducers and stress stimulation. In vitro studies show that ERK, p38 MAP kinase, and Jun N-terminal kinase can phosphorylate and activate MAPKAPK3, indicating its role in integrating mitogen and stress response signaling.
MAPKAPK3 interacts with, phosphorylates, and represses E47, a basic helix-loop-helix transcription factor involved in tissue-specific gene expression and cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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