Thermo Fisher Scientific CKAP2 Polyclonal Antibody
Human CKAP2 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC(P), ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보존됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human CKAP2. Recombinant protein control fragment (Product #RP-89675) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2639834 |
Product Specific Information
Immunogen sequence:
PPIRSHHSNT RDTVKQGISR TSANVTIRKG PHEKELLQSK TALSSVKTSS SQGIIRNKTL SRSIASEVVA RPASLSNDKL MEKSEPVDQR RHTAGKAIVD SRSAQPKETS EERKARLSEW KAGKGR
Highest antigen sequence identity to the following orthologs:
- Mouse: 51%
- Rat: 49%
Target Information
Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins. It may have a fundamental role in embryonic processes.
N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. It also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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