Thermo Fisher Scientific Bu-1b Monoclonal Antibody (5-11G2), FITC
닭 Bu-1b 항원을 특이적으로 인식하는 단클론 항체로, FITC로 표지되어 유세포분석에 적합합니다. 498/517 nm의 여기/방출 파장을 가지며, 4°C 암소 보관이 권장됩니다. 연구용으로만 사용 가능합니다.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: 1 µg/10^6 cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5-11G2 |
| Immunogen | Bursa cells from two- to five-week-old CHA (Bu-1b) strain chickens |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4, with proprietary stabilizer |
| Contains | <0.1% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745716 |
Additional Formats
- Unconjugated (Cat. No. MA5-28759)
- Biotin (Cat. No. MA5-28756)
- PE (Cat. No. MA5-28758)
- Custom conjugation available upon request
Target Information
Bu-1b, a product of the Bu-1b allele, is expressed on the cell surface as a disulfide-linked homodimer.
In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on:
- 85–90% of bursal cells
- 2–8% of thymocytes
- 15–27% of spleen cells
- 2–18% of peripheral blood cells
It is also expressed on subsets of macrophages and monocytes but not on granulocytes, erythrocytes, or thrombocytes.
In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion.
The monoclonal antibody 5-11G2 reacts with lymphoid cells from CHA and H.B14Ab strains but does not react with cells from H.B14Aa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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