Thermo Fisher Scientific Bu-1b Monoclonal Antibody (5-11G2), FITC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/10^6 cells

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Product Specifications

Species Reactivity

Chicken

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

5-11G2

Immunogen

Bursa cells from two- to five-week-old CHA (Bu-1b) strain chickens

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• Biotin
• PE
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4, with proprietary stabilizer

Contains

<0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2745716

Target Information

Bu-1b, a product of the Bu-1b allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes but not on granulocytes, erythrocytes, or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion. The monoclonal antibody 5-11G2 reacts with lymphoid cells from CHA and H.B14Ab strains but does not react with cells from H.B14Aa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.