Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), NovaFluor Violet 690, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), NovaFluor Violet 690, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV/Violet series
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue/Red/Violet/Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright series
• Custom conjugation available

Product Specific Information

The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all stages of development except plasma cells. Follicular dendritic cells also express CD19. CD19 associates with CD21, CD81, Leu13, and MHC class II forming a multimolecular complex with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and B cell proliferation. The SJ25C1 antibody and HIB19 recognize overlapping epitopes.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Use 5 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL of CellBlox Plus Blocking Buffer per sample, regardless of the number of NovaFluors used.
• For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures designed to minimize spillover and spread.
Excitation: 408 nm | Emission: 691 nm | Laser: 405 nm (Violet)

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on 100% of peripheral B cells and certain myeloid leukemia cells. CD19 acts as a signal-amplifying coreceptor for the B cell receptor (BCR) and regulates normal and malignant B cell proliferation. Mutations cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures.

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