Invitrogen™

NucRed™ Live 647 ReadyProbes™ Reagent

NucRed Live 647 ReadyProbes Reagent is a bright, far-red, cell-permeant nuclear stain for live or fixed cells. It is provided자세히 알아보기

NucRed Live 647 ReadyProbes Reagent is a bright, far-red, cell-permeant nuclear stain for live or fixed cells. It is provided as a room temperature-stable solution in a convenient-to-use dropper bottle. Just add two drops per ml to stain your cells.

Provided at 12.5X concentration and formulated with 1% DMSO, PowerLoad solubilizing agent, and HBSS buffer. Also available as HCS Nuclear Mask Deep Red Stain at 250X concentration in DMSO.

• No need to dilute, weigh, or pipette
• Convenient dropper bottle—just use two drops per mL
• Stable at room temperature—keep handy at your scope or cell culture area
• Excited at 638 nm when bound to DNA, with an emission maximum at 686 nm

See other ReadyProbes reagents for cell staining
See other nuclear stains for imaging

Cell imaging applications
The far-red fluorescence emission makes it ideal for use in combination with blue, green, and red fluorophores, such as Hoechst, GFP/FITC, and Texas Red.

Suggestions for use
• NucRed Live 647 ReadyProbes Reagent may be added directly to cells in full media, or buffer solutions.
• In most cases, 2 drops/mL and an incubation of 15 to 30 minutes will give bright nuclear staining; however, more or fewer drops can be added for optimal staining intensity.
• In most cases, staining intensity increases with time if cells are not washed prior to imaging.
• It is detected through a far-red filter, such as a Cy5 filter, or a Cy5 Light Cube for EVOS imaging systems.

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

사양

색상Far-Red

여기 파장 범위638⁄686

염료 유형Cell-Permeant

용도(장비)Confocal Microscope, Fluorescence Microscope, Flow Cytometer

수량6 dropper bottle(s) kit

검출 방법Fluorescence

방출Visible

형태Liquid

제품라인NucRed™, ReadyProbes™

라벨 유형Fluorescent Dye

제품 유형Nucleic Acid Stain

SubCellular LocalizationNucleus

Unit SizeEach