Applied Biosystems™

MagMAX™-96 Viral RNA Isolation Kit

The MagMAX-96 Viral RNA Isolation Kit is for the isolation of viral RNA and DNA from diverse sample types (e.g.,자세히 알아보기

The MagMAX-96 Viral RNA Isolation Kit is for the isolation of viral RNA and DNA from diverse sample types (e.g., environmental samples and biofluids). Nucleic acids purified using this kit are ideal for PCR and RT-PCR applications. For a NVSL-validated version of this kit, please see the MagMAX-96 AI⁄ND Viral RNA Isolation Kit.

Benefits of MagMAX magnetic bead-based purification:
Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20–50 μL of nuclease-free water, concentrating RNA from large, dilute samples.

Optimized for isolation of virus from biofluids
The MagMAX Viral RNA Isolation Kit has been optimized for use with biological fluids and cell-free samples such as serum, plasma, swabs, and cell culture media. These samples often present unique challenges when purifying RNA, including low viral titers, large volumes to be processed, and sample complexity. Without the need for organic solvents or RNA precipitation, the MagMAX Viral RNA Isolation Kit eliminates the problems often encountered with other RNA isolation methods such filter clogging, large elution volumes, and inconsistent yields. With this kit, DNA and RNA input levels ranging from 20 copies to 25 x 106 copies have been quantitatively recovered from plasma, serum, and milk. And even with less sample input, pathogen detection was as sensitive (equivalent CTs) or more sensitive (lower CTs) using nucleic acid isolated with the MagMAX kit when compared to nucleic acid isolated using another company`s product.

Accessory Products:
This kit is optimized for automated nucleic acid purification using KingFisher magnetic particle processors. This kit can also be used for manual nucleic acid purification using a 96-well magnetic ring stand. The Magnetic Stand-96 (Cat. No. AM10027) and the 96-well Magnetic Ring Stand (Cat. No. AM10050) are designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates. The Magnetic-Ring Stand pellets the magnetic beads in a circle shape, enabling easy removal of supernatant by a robot or multichannel pipettors.

사양

형식96-well Plate

Isolation TechnologyMagnetic Bead

정제 시간45 min.

샘플 종류Cell-free Biological Fluids, Liquid Samples (e.g. Serum), Viral Samples

최종 제품 유형Viral DNA⁄RNA

고처리량 호환성High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols

용도(장비)MagMAX™ Express-96 Magnetic Particle Processor, KingFisher 96, MagMAX™ Express-96 Standard Magnetic Particle Processor

용도(애플리케이션)Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting

반응 수96

제품라인MagMAX™

수량96 preps

스케일Midi

시작 물질 양Up to 50μl of sample

TargetViral DNA/RNA

수율5 ng

Unit SizeEach