Thermo Fisher Scientific Histone H3 Polyclonal Antibody
Histone H3 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, ICC/IF, ChIP 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 시료에 반응하며, 고순도의 친화 크로마토그래피 정제 항체입니다. 액상 형태로 4°C 또는 -20°C에서 안정적으로 보관 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 |
| ChIP assay (ChIP) | 1.5 µg / 1×10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around aa.8–12 (R-K-S-T-G) derived from Human Histone H3.1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2635033 |
Product Specific Information
The antibody detects endogenous levels of total Histone H3 protein.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translationally, histones are modified in various ways to change chromatin structure or enable transcription factor binding. The N-terminal tail of histone H3 can undergo multiple post-translational modifications, including methylation, acetylation, and phosphorylation, which influence cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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