Thermo Fisher Scientific eBioscience TMB Solution (1X)

Invitrogen™ eBioscience™ TMB Solution (1X)

The 1X eBioscience™ TMB Solution is a sensitive chromogenic substrate supplied as a single ready-to-use solution.
TMB (3,3’,5,5’-Tetramethylbenzidine) is a substrate for horseradish peroxidase (HRP) and is recommended for use with Ready-SET-Go! ELISA kits.

During the enzymatic reaction, the TMB substrate produces a blue color that can be read on a spectrophotometer at 650 nm.
This kinetic reaction can be stopped using 1M phosphoric acid, resulting in a color change to yellow that can be read at 450 nm.

Reported Application

• ELISA

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