Thermo Fisher Scientific p53 Monoclonal Antibody (G053.1)

Applications

• Immunohistochemistry (Paraffin) (IHC (P)): 1:100–1:200

Product Specifications

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation. Upon activation, p53 induces transcription of genes such as p21waf1, leading to G1 or G2/M arrest. Structurally, p53 includes:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerization domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is critical for activation and stabilization, while Ser392 phosphorylation is associated with tumor formation. p53 plays a key role in preventing proliferation of damaged cells and functions as an anticancer factor. It is also linked to aging, oxidative stress, and memory-related processes. Normally expressed at low levels, p53 is highly expressed in many transformed cell lines, contributing to malignancy. Mutations in the TP53 gene lead to loss of tumor suppressor activity and are implicated in various cancers, including hereditary cancer syndromes such as Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.