Thermo Fisher Scientific HLA-DP/-DQ/-DR (MHC II) Monoclonal Antibody (CR3/43)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1-2 µg/mL

-

Immunocytochemistry (ICC/IF)

1-2 µg/mL

-

Flow Cytometry (Flow)

1-2 µg/ 10^6 cells

-

ELISA (ELISA)

2-4 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

CR3/43

Immunogen

Cells from human tonsil if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HLA-DR/DP/DQ,uniProtId:P01911-1,ncbiNodeId:9606,antigenRange:1-266,antigenLength:266,antigenImageFileName:3115-MSM3-P1ABX_HLA-DRDPDQ_P01911-1_House_mouse.svg,antigenImageFileNamePDP:3115-MSM3-P1ABX_HLA-DRDPDQ_P01911-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:HLA-DR/DP/DQ,uniProtId:P01920-1,ncbiNodeId:9606,antigenRange:1-261,antigenLength:261,antigenImageFileName:3115-MSM3-P1ABX_HLA-DRDPDQ_P01920-1_House_mouse.svg,antigenImageFileNamePDP:3115-MSM3-P1ABX_HLA-DRDPDQ_P01920-1_House_mouse_PDP.jpeg,sortOrder:2},{targetFamily:HLA-DR/DP/DQ,uniProtId:P04440-1,ncbiNodeId:9606,antigenRange:1-258,antigenLength:258,antigenImageFileName:3115-MSM3-P1ABX_HLA-DRDPDQ_P04440-1_House_mouse.svg,antigenImageFileNamePDP:3115-MSM3-P1ABX_HLA-DRDPDQ_P04440-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

MHC class II molecules are transmembrane glycoproteins composed of an alpha chain (36 kDa) and a beta chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They therefore have a critical role in the initiation of the immune response.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.