Invitrogen™

Chromatin Condensation & Membrane Permeability Dead Cell Apoptosis Kit with Hoechst 33342, YO-PRO™-1, and PI dyes, for flow cytometry

This product detects apoptotic cells with changes in nuclear chromatin condensation and plasma membrane permeability, using three nucleic acid stains:자세히 알아보기

This product detects apoptotic cells with changes in nuclear chromatin condensation and plasma membrane permeability, using three nucleic acid stains: UV-excitable Hoechst 33342, green fluorescent YO-PRO™ dye, and propidium iodide. The YO-PRO™ dye can enter apoptotic cells, whereas red-fluorescent propidium iodide (PI) cannot. Thus after staining with YO-PRO™-1 dye and PI, apoptotic cells show green fluorescence, and dead cells show primarily red fluorescence and some green fluorescence. Blue fluorescent Hoechst 33342 brightly stains the condensed chromatin of apoptotic cells and more dimly stains the normal chromatin of live cells. The staining pattern resulting from the simultaneous use of these three dyes makes it possible to distinguish normal, apoptotic and dead cell populations by flow cytometry or fluorescence microscopy.

사양

수량1 kit

형식Kit

제품 유형Dead Cell Apoptosis Kit

유세포분석기 레이저 라인488 nm

여기/방출YO-PRO™-1: 491/509 cm, Hoechst 33342: 350/461 cm, PI: 535/617 cm

반응 수200 Reactions

배송 조건Room Temperature

제품라인Vybrant™, YO-PRO™

용도(애플리케이션)Flow Cytometry

콘주게이트Hoechst 33342, Propidium Iodide, YO-PRO-1

용도(장비)Flow Cytometer

형태Solution

Unit Size1 kit