Thermo Fisher Scientific Sgt-1A Monoclonal Antibody (5B12F5)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5B12F5

Immunogen

A recombinant protein Sgt A

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS with proprietary stabilizer

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Positive control: Testis

Cellular location: Nucleus.

Target Information

This gene encodes a highly conserved nuclear protein involved in kinetochore function and required for the G1/S and G2/M transitions. Human Sgt1 (Suppressor of the G2 allele of Skp1) has been found to have two isoforms: Sgt-1A and Sgt-1B. This protein interacts with heat shock protein 90. Sgt1 protein translocates to the nucleus due to heat shock and the Ca(2+)-bound form of S100A6 is required for Sgt1 translocation. S100A6-Ca(2+)-mediated Sgt1 dephosphorylation promotes its nuclear translocation, most likely due to disruption of the Sgt1-Hsp90 complex. SUGT1(Sgt-1) protein expression was shown in lung , testis, liver, and brain. It is overexpressed in human colorectal cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.