Thermo Fisher Scientific Histone H3, phosphorylated (pSer10) Recombinant Rabbit Monoclonal Antibody (PHH3, 471R)
Histone H3 (pSer10)에 특이적인 재조합 토끼 단일클론 항체로, 인간 시료에 반응하며 IHC(P) 실험에 적합합니다. HEK293 발현 시스템 기반, Protein A/G 정제, PBS 버퍼에 저장되며 연구용으로만 사용됩니다.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P))
Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PHH3, 471R |
| Immunogen | A synthetic peptide corresponding to (ARK-pS-TGGKAPRKQLc) of Phosphohistone H3 (phospho S10). The antigen corresponds to amino acid range 8–21 of the target protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 min.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells.
H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome.
Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translationally, histones are modified in various ways to change chromatin structure or allow binding of specific transcription factors.
The N-terminal tail of histone H3 protrudes from the nucleosome core and can undergo modifications such as methylation, acetylation, and phosphorylation affecting cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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