Thermo Fisher Scientific GAPDHS Polyclonal Antibody
GAPDHS 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 사용 가능. 인간 및 마우스 반응성, 고순도 항원 친화 크로마토그래피 정제. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:10,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 161–391 of GAPDHS (Uniprot ID: O14556) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.91 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552199 |
Product Specific Information
- Recommended positive controls: MCF-7, NIH-3T3, mouse testis
- Predicted reactivity: Mouse (90%), Rat (90%), Zebrafish (82%), Bovine (93%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme that catalyzes a key step in glycolysis — the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. GAPDH is highly expressed and often used as a reference in mRNA and protein expression studies. It interacts with proteins such as amyloid precursor protein (linked to Alzheimer’s disease) and Huntingtin (linked to Huntington’s disease). GAPDH also associates with actin and tubulin, and may play a role in apoptosis regulation, migrating to the nucleus during apoptotic processes.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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