Thermo Fisher Scientific Histone H3 Polyclonal Antibody
Histone H3 단백질을 인식하는 Thermo Fisher의 다클론 항체로, Western blot, IHC, ICC, IP, ChIP 등 다양한 응용에 사용 가능. Rabbit IgG 기반, 고순도 항원 친화 크로마토그래피 정제. 4°C 또는 -20°C 보관 권장.
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- PA531954
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:10,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 1 publication |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
| ChIP Assay (ChIP) | Assay-dependent | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Fruit fly, Fungi, Hamster, Human, Mouse, Non-human primate, Plant, Rat |
| Published Species | Human, Yeast |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 1–66 of Histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.82 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2549427 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, PC-12, Rat2, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability.
Post-translational modifications of histone H3, such as methylation, acetylation, and phosphorylation, influence chromatin structure and transcription factor binding. The N-terminal tail of histone H3 extends from the nucleosome core and undergoes multiple types of modifications affecting cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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