Thermo Fisher Scientific MCM7 Monoclonal Antibody (MCM7, 1466)

Applications

Western Blot (WB)

• Tested Dilution: 1–2 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.

Target Information

MCM7 encodes a protein that is one of the highly conserved mini-chromosome maintenance proteins (MCM) essential for the initiation of eukaryotic genome replication.
The hexameric complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and recruitment of other DNA replication-related proteins.
The MCM complex consisting of MCM7, MCM2, MCM4, and MCM6 possesses DNA helicase activity and may act as a DNA unwinding enzyme.
Cyclin D1-dependent kinase CDK4 is found to associate with MCM7 and may regulate its interaction with the tumor suppressor protein RB1/RB.
Alternatively spliced transcript variants encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.