Thermo Fisher Scientific HIST1H2BH Polyclonal Antibody
Histone H2B 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF에 사용 가능. 인간 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. PBS/glycerol buffer에 보관하며 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from internal region of human Histone H2B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS (pH 7.4) with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2635317 |
Product Specific Information
This antibody detects endogenous levels of total Histone H2B protein.
Target Information
In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3, and H4, and associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H1 is required for the condensation of nucleosome chains into higher-order structures. Phosphorylation of Histone H1 is thought to be involved in this process, although the exact nature of this role has yet to be elucidated. Evidence suggests that Histone H1 is part of a general repressor mechanism for stable repression of transcription, but it can also activate transcription of specific genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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