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Thermo Fisher Scientific Acetylcholinesterase Fluorescent Activity Kit, 2 x 96 Tests

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혈청, 혈장 및 적혈구막 시료에서 아세틸콜린에스터레이스 활성을 정량 및 검출하는 형광 기반 연구용 키트. 2 x 96웰 포맷으로 완전 구성된 시약 포함. 390 nm/510 nm 형광 판독기 사용. 다양한 종에 적용 가능.

카탈로그번호: EIAACHEF
브랜드: Thermo Fisher Scientific
카테고리: Enzymatic Tests
판매단위: pk

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Thermo Fisher Scientific EIAACHEF Acetylcholinesterase Fluorescent Activity Kit, 2 x 96 Tests Each pk판매 단위 pk · 1~2일 내 출고

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922,800원VAT 포함 1,015,080원

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Thermo Fisher Scientific · Thermo Fisher Scientific Acetylcholinesterase Fluorescent Activity Kit, 2 x 96 Tests

Invitrogen™ Acetylcholinesterase Fluorescent Activity Kit

The Acetylcholinesterase Fluorescent Activity Kit is a research-use-only fluorescent assay designed for the quantification and detection of acetylcholinesterase (AChE) activity in serum, plasma, and erythrocyte membrane samples.

This complete, ready-to-use kit includes:

Black 96-well plate(s)
Acetylcholinesterase standard (1,000 mU/mL)
Acetylcholinesterase substrate
Acetylthiocholine iodide
Additional reagents for assay performance

A fluorescence 96-well microplate reader capable of detecting emission at 510 nm (excitation at 390 nm) is required.

Performance Characteristics

항목	내용
Assay Type	Fluorescent Activity Kit
Sample Types	Serum, Plasma, Erythrocyte Membrane Samples
Sensitivity	0.218 mU/mL
Standard Curve Range	6.25–100 mU/mL
Reactivity	Species Independent

Background

Acetylcholine (ACh) is an essential neurotransmitter in both the central and peripheral nervous systems. It mediates chemical neurotransmission through nicotinic and muscarinic receptors found in neurons, ganglia, heart, smooth muscle fibers, and glands.

Acetylcholinesterase (AChE), encoded by the single AChE gene, exhibits structural diversity due to alternative mRNA splicing and post-translational modifications.

The hydrophilic form predominates in brain and muscle tissues, forming disulfide-linked oligomers with structural subunits.
The erythroid form contains a cleavable peptide with a GPI anchor, associating with membranes via phosphoinositide moieties.

This assay is validated for serum, EDTA and heparin plasma, and solubilized RBC ghosts from multiple species.

Assay Principle

The kit quantitatively measures AChE activity using a proprietary non-fluorescent molecule that binds covalently to the thiol product of the enzymatic reaction between AChE substrate and enzyme.
This reaction yields a fluorescent product detectable at 510 nm (excitation 390 nm) using a fluorescence plate reader.

A human AChE standard is included to generate a standard curve for accurate quantification.
The assay is suitable for:

Diluted serum and plasma
RBC ghosts
Extracted tissue samples
Cell lysates

AChE Unit Definition

One unit hydrolyzes 1.0 μmol of acetylthiocholine iodide per minute at pH 7.4 and 37°C.

제품 사양

항목	내용
이뮤노어세이 키트 형식	Assay Activity Kit
샘플 종류	Plasma, Serum
용도(장비)	Microplate Reader
수량	2 x 96 Tests
배송 조건	Approved for shipment on Wet or Dry Ice
타겟 키트 이름 지정	Acetylcholinesterase
Unit Size	Each