Thermo Fisher Scientific UCP-1 Polyclonal Antibody
UCP-1 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC, Flow cytometry, ELISA에 사용 가능. 다양한 종(Goat, Human, Mouse, Rat)에 반응하며, Protein A로 정제된 1 mg/mL 액상 항체. 연구용으로 갈색 지방조직의 열생성 관련 단백질 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Goat, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human UCP-1, amino acids 101–200 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Mitochondrial uncoupling proteins (UCPs) are members of the mitochondrial anion carrier protein family. UCPs separate oxidative phosphorylation from ATP synthesis, dissipating energy as heat (mitochondrial proton leak). They facilitate anion transfer across the mitochondrial membrane and reduce mitochondrial membrane potential in mammalian cells.
UCP-1 is specifically expressed in brown adipose tissue, which functions to produce heat.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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