
Thermo Fisher Scientific TAP Tag Polyclonal Antibody
TAP 태그 단백질 검출용 Rabbit Polyclonal 항체로, WB, IP, ChIP 등 다양한 응용에 적합합니다. TEV 절단 후 TAP C-말단 펩타이드로 면역화되었으며, 항원 친화 크로마토그래피로 정제되었습니다. 비보존액 형태로 4°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1000 | View 83 publications |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Immunoprecipitation (IP) | Assay-dependent | View 7 publications |
| ChIP assay (ChIP) | Assay-dependent | View 12 publications |
| Miscellaneous PubMed (Misc) | - | View 5 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | BBS, pH 8.4 |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10709700 |
Product Specific Information
- CAB1001 detects TAP.
- Successfully used in Western Blot, Immunoprecipitation (IP), and ChIP applications.
- Immunogen: KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage.
- TAP adds approximately 20 kDa to the size of the protein.
Target Information
Epitope tags allow researchers to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize low abundance or poorly immunogenic proteins when specific antibodies are unavailable.
Tandem affinity purification (TAP) is an affinity-based purification method for isolating TAP-tagged proteins along with associated partners. The TAP tag typically includes a calmodulin binding peptide (CBP), a tobacco etch virus (TEV) protease cleavage site, and Protein A. This technique is valuable for analyzing in vivo protein–protein interactions.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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