Thermo Fisher Scientific TAP Tag Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

View 83 publications 83 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

View 7 publications 7 publications

ChIP assay (ChIP)

Assay-dependent

View 12 publications 12 publications

Miscellaneous PubMed (Misc)

-

View 5 publications 5 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

BBS, pH 8.4

Contains

no preservative

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10709700

Product Specific Information

CAB1001 detects TAP.

CAB1001 has been successfully used in Western Blot, IP, and ChIP applications

The CAB1001 immunogen is a KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage.

TAP adds about 20 kDa to the size of the protein.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the TAP tag (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.