Invitrogen™

SuperScript™ IV One-Step RT-PCR System

The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.

The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance. With this convenient one-step formulation plus gene-specific primers, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a single tube.

사양

검출 방법Gel Electrophoresis

형식Kit

GC-Rich PCR PerformanceHigh

PCR 방법1-step RT-PCR

중합효소Platinum SuperFi

반응 속도10 min.

기술1-step RT-PCR

최적 반응 온도45°C to 60°C

수량Each

역전사 효소SuperScript IV

리보뉴클레아제 H 활성Reduced

배송 조건Dry Ice

최종 제품 유형PCR Amplified cDNA

핫 스타트Built-In Hot Start

반응 수25 Reactions

반응 형식Master Mix

시약 유형Reverse Transcription

크기(최종 제품)Up to 14 kb

시작 물질RNA

포함Kit only

Unit SizeEach