Thermo Fisher Scientific Phospho-ETS1 (Ser251) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

Immunohistochemistry (IHC)

• Tested Dilution: 1:50–1:100

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: Assay-Dependent

Product Specifications

Product Specific Information

Phospho-ETS1 (Ser251) Polyclonal Antibody detects endogenous levels of ETS1 only when phosphorylated at Ser251.

Target Information

ETS1 (c-Ets-1; v-ets erythroblastosis virus E26 oncogene homolog 1; ETS-1; EWSR2; p54) is a 441 amino acid protein and a member of the DNA-binding ETS protein family.
It is a multifunctional protein with:

• N-terminal pointed domain
• Central transactivation domain
• Two auto-inhibitory domains flanked by a conserved C-terminal Ets domain

ETS1 interacts with factors such as PEBP2alphaA and p300/CBP, mediating transactivation of Osteopontin, Presenilin-1, Stromelysin, and Collagenase.
Activated by Ras/MAPK signaling, its expression is upregulated in resting T-cells.
Activity modulation occurs via AP-1, Sp-1, c-myb, MafB, and Sp100 (activator in nuclear bodies).
ETS1 also interacts with EAP1/Daxx (repression of MMP1 and BCL2 transactivation), STAT6 (modulation of Socs-1 expression induced by IL-4), and GATA3 (positive regulation of Tax-1 dependent IL-5 expression).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.