Thermo Scientific™

Clean-Blot™ IP Detection Reagent (HRP)

Thermo Scientific Clean-Blot IP Detection Reagent is a horseradish peroxidase conjugate that is optimized for post-immunoprecipitation Western blot detection of자세히 알아보기

Thermo Scientific Clean-Blot IP Detection Reagent is a horseradish peroxidase conjugate that is optimized for post-immunoprecipitation Western blot detection of primary antibodies without interference from denatured IP antibody fragments.

The Clean-Blot IP Reagent allows trouble-free Western blot detection of target proteins following IP assays (immunoprecipitation). It works by specifically binding to functional primary antibodies (whole IgG) without also binding to fragments of the IP antibodies, which usually accompany the immunoprecipitated protein through electrophoresis and membrane transfer. The Clean-Blot IP Reagent and Kit eliminate detection-interference from both heavy-chain (approx. 50kDa) and light-chain (25kDa) IgG-fragments of antibodies used for the initial immunoprecipitation assay.

Features of the Clean-Blot IP Detection Reagent:

• Universal—bind and detect most species, IgG subclasses and isotypes of primary antibodies that are commonly used for Western blotting
• Compatible—effective with IP assays performed using Protein A, Protein G, or anti-IgG agarose beads and any blocking buffer
• Cost-effective—eliminates the cost and extra work associated with covalently immobilizing IP antibodies as a means of overcoming Western blot interference
• Flexible—horseradish peroxidase (HRP) reagent for detection with chemiluminescent, fluorescent or colorimetric substrates
• Easy to use—no need to change the Western blotting protocol; simply replace conventional secondary HRP conjugate with the Clean-Blot IP Detection Reagent
• Unobstructed detection—clear Western blot results for immmunoprecipitation assays without significant interference from denatured IgG bands

Related Products
Clean-Blot™ IP Detection Kit (HRP)

사양

설명Clean-Blot IP Detection Reagent (HRP)

제품 유형IP Detection Reagent (HRP)

검출 방법Chemiluminescence, Fluorescence, Colorimetric

형태Liquid

용도(애플리케이션)Western Blot

제품라인Pierce™

수량2.5 mL

콘주게이트HRP

반응성All species

Unit SizeEach