Thermo Fisher Scientific Human IFN-beta Recombinant Protein, PeproTech
인간 유래 IFN-beta 재조합 단백질로, CHO 세포에서 발현된 고순도(≥95%) 제품입니다. 세포 독성 분석을 통해 활성이 검증되었으며, 바이러스 감염 및 면역 반응 연구에 적합합니다. 동결건조 형태로 제공되며 -20°C에서 보관합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| ELISA | - | View 2 publications |
| Functional Assay | Assay-dependent | View 1 publication |
| Inhibition Assays | - | View 1 publication |
| In vitro Assay | - | View 92 publications |
| Miscellaneous PubMed | - | View 18 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Published species | Human, Mouse, Pig |
| Expression System | CHO cells |
| Amino acid sequence | MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN |
| Molecular weight | 20 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥ 95% by SDS-PAGE gel and HPLC analyses |
| Endotoxin concentration | <1 EU/µg |
| Activity | Determined by a cytotoxicity assay using human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10^7 units/mg |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage conditions | -20°C |
Product Specific Information
- Catalog No. 300-02BC-1MG is supplied as 2 × 500 µg (300-02BC-500UG).
- Recombinant Human IFN-beta is a 20.0 kDa protein containing 166 amino acid residues.
- Due to glycosylation, IFN-beta has an approximate MW of 22.3 kDa based on SDS-PAGE and Mass Spectrometry.
- Human IFN-beta (Catalog Number 300-02BC) has replaced Human IFN-beta (Catalog Number 300-02B).
- Shipped at ambient temperature. For storage, handling, and reconstitution information, refer to the lot-specific Certificate of Analysis.
Target Information
Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by natural IFN-producing cells (IPCs) as part of the host immune response. These interferons can also inhibit tumor development. IFN-beta binding activates tyrosine kinases Jak1 and Tyk2, leading to phosphorylation of STAT transcription factors and expression of immune response genes. Members of the toll-like receptor (TLR) family are known to stimulate IFN-beta production. Clinically, IFN-beta is used for the treatment of tumors, infections, and multiple sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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