Thermo Scientific™

CellSensor™ c-Fos-bla ME-180 Cell Line

The CellSensor™ c-fos-bla ME-180 cell line contains a beta-lactamase reporter gene under control of the c-fos response element stably integrated자세히 알아보기

The CellSensor™ c-fos-bla ME-180 cell line contains a beta-lactamase reporter gene under control of the c-fos response element stably integrated into ME-180 cells. To construct this cell line, the c-fos-bla construct was transduced into ME-180 cells by lentivirus. Flow cytometry was used to isolate cells responsive to epidermal growth factor (EGF). This cell line is validated for DMSO tolerance, cell number, stimulation time, substrate loading time, as well as Z` and EC50 concentrations for EGF. The CellSensor™ c-fos-bla ME-180 cell line is responsive to EGF, HGF, IL-6, OSM, TNFα, IL-1-alpha; and PMA/Thaps, and can be used to probe the JNK/P38/MAPK and JAK/STAT signaling pathways. This cell line can be adapted for high-throughput screening of agonist or antagonist compound libraries. Candidate drugs can also be tested for dose response against this cell line. Academic and non-profit customers, please inquire for special pricing.

사양

어세이 입력Cell-based beta lactamase reporter gene

셀 상태Dividing Cells

제품라인CellSensor™

수량1 Vial

타겟 입력STAT1, STAT3, JAK1, EGFR, ERK1/2, p38, MAPK pathway, RAF, JAK2, c-MET, JNK, JAK/STAT (IFN gamma, IL-6/OSM) pathway, RON, MEK

System TypeCellSensor™

Druggable TargetSignaling Pathway

Unit SizeEach