Thermo Fisher Scientific GeneBLAzer M1 CHO-K1 DA Assay Kit
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| 카탈로그 번호 | CAS 번호 | 설명 | 상태 | 단위 | 판매가 | 할인가 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
| K1365 | - | Thermo Fisher Scientific K1365 GeneBLAzer M1 CHO-K1 DA Assay Kit Each pk | 재고문의 | pk | 0원 | - | 0원 | |
다른 상품 둘러보기
Thermo Scientific™
GeneBLAzer™ M1 CHO-K1 DA Assay Kit
This cell line is available as dividing cells by requesting a quote for K1230 from dividingcells@invitrogen.comGeneBLAzer™ M1 CHO-K1 DA(Division Arrested)자세히 알아보기
This cell line is available as dividing cells by requesting a quote for K1230 from dividingcells@invitrogen.com
GeneBLAzer™ M1 CHO-K1 DA(Division Arrested) cells and M1-NFAT-bla CHO-K1 cells contain the human Acetylcholine (muscarinic) subtype 1 receptor (M1) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. No. K1078) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element. Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.
DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both M1 CHO-K1 DA cells and M1-NFAT-bla CHO-K1 cells are functionally validated for Z`-factor and EC50 concentrations of carbachol, (Figure 1). In addition, M1-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
The Musacarinic 1(M1) Receptor is localized principally in the cortical and hippocampal regions of the brain and has been implicated in various neurological conditions including depression, anxiety, and sleep disorders.
사양
등록 번호NM_000738
어세이 입력Cell-based beta lactamase reporter gene
셀 상태Division-Arrested Cells
검출 방법Fluorescence
유전자 ID(Entrez)1128
유전자 기호M1
개체군 상태Single Cell Clone Origin
제품라인GeneBLAzer™
수량1 plate
리포터 유전자BLA (Beta-Lactamase)
타겟 입력M1
세포주CHO-K1
System TypeGeneBLAzer™
Druggable TargetGPCR
Unit SizeEach
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