Thermo Fisher Scientific N4BP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human N4BP1. Recombinant protein control fragment (Product #RP-98671). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:N4BP1,uniProtId:O75113-1,ncbiNodeId:9606,antigenRange:419-517,antigenLength:896,antigenImageFileName:PA5-59323_N4BP1_O75113-1_Rabbit.svg,antigenImageFileNamePDP:PA5-59323_N4BP1_O75113-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2644410

Product Specific Information

Immunogen sequence: NELTTDSTPK KTQAHTQQNM VEKFSQLPFK VEAKPCTSNC RINTFRTVPI EQKHEVWGSN QNYICNTDPE TDGLSPSVAS PSPKEVNFVS RGASSHQPR

Highest antigen sequence identity to the following orthologs: Mouse - 68%, Rat - 67%.

Target Information

Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.