Thermo Scientific™

AjuI (5 U/μL)

5↓N7G A A N7T T G G N11↓ 33↑N12C T T N7A A C C N6↑ 5Thermo Scientific자세히 알아보기

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5`			↓	N7	G	A	A	N7	T	T	G	G	N11	↓		3`
3`			↑	N12	C	T	T	N7	A	A	C	C	N6	↑		5`

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Thermo Scientific AjuI restriction enzyme recognizes ^(7/12)GAA(N)7TTGG(11/6)^ sites and cuts best at 37°C in R (+SAM) buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: Complete cleavage of some substrates with AjuI is difficult to achieve. AjuI concentration is determined by the maximum cleavage level achieved when no change in the fragmentation pattern is observed with further enzyme increase. AjuI produces double-strand cuts on both sides of the interrupted recognition site. In certain sequence contexts, the cleavage position may be shifted by one base pair. However, the cleavage position indicated above will predominate.

사양

농도5 U/μL

메틸화 민감도Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive

효소Aju I

함께 사용가능한 버퍼10X Buffer R

열 불활성화에 대한 민감성Yes

최적 반응 온도37°C

Type IIS REYes

수량100 units

제품 유형Restriction Enzyme

연구 카테고리Traditional Cloning

Unit SizeEach