Thermo Fisher Scientific APC1 Polyclonal Antibody
인간 APC1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, ELISA, IP에 사용 가능. 친화 크로마토그래피로 정제된 액상 항체이며, -20°C에서 보관. 세포주기 조절 및 APC/C 복합체 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 - 1:2,000 |
| ELISA | 1:5,000 - 1:20,000 |
| Immunoprecipitation (IP) | 1:500 - 1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to an internal region near amino acids 350–375 of Human APC1 (Swiss Prot accession Q9H1A4) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
- This affinity purified antibody is directed against human APC1 at the S355 residue.
Target Information
Cell cycle regulated protein ubiquitination and degradation within subcellular domains is essential for normal mitosis progression.
APC1 (also known as mitotic checkpoint regulator, MCPR) is a conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase controlling mitosis and G1 phase progression.
APC/C degrades anaphase inhibitors, mitotic cyclins, and spindle-associated proteins to ensure proper mitotic sequence.
APC/C components’ mRNA and protein levels are expressed at similar levels across tissues and cell lines, indicating their function as a complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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