Thermo Fisher Scientific Histone H3.3 Polyclonal Antibody
Histone H3.3 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. WB, IHC, ICC/IF, ChIP 등 다양한 응용에 적합. Human, Mouse, Rat 등과 반응하며 항원 친화 크로마토그래피로 정제됨. 장기 보관 시 -20°C에서 보관 권장.
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Thermo Fisher Scientific Histone H3.3 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| ChIP assay (ChIP) | 1.5 µg / 1×10⁶ cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Full-length human Histone H3.3B recombinant protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.16 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11156178 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain, Rat brain
- Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Chicken (100%), Chimpanzee (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of H2A, H2B, H3, and H4. The chromatin fiber is compacted through linker histone H1 interaction, forming higher-order chromatin structures.
This gene encodes a member of the histone H3 family, located separately from other H3 genes on chromosome 6p22–p21.3. Transcripts lack polyA tails and contain a palindromic termination element.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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