Thermo Fisher Scientific Tyrosine Hydroxylase Monoclonal Antibody (1B8D2)
Tyrosine Hydroxylase 단백질을 표적하는 mouse monoclonal antibody로, WB, IHC, ICC/IF, Flow, ELISA 등 다양한 응용에 적합. Human 및 Rat 시료에 반응하며, Protein G로 정제된 고순도 항체. 신경전달물질 합성 관련 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1B8D2 |
| Immunogen | Purified recombinant fragment of human TH (aa: 44–208) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898553 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
Tyrosine hydroxylase (TH) is an enzyme involved in the synthesis of catecholamine neurotransmitters such as dopamine, epinephrine, and norepinephrine. In all species, catecholamine synthesis is regulated by the interaction of TH with the cofactor tetrahydrobiopterin (BH4), which binds to the catalytic domain to activate the enzyme.
Four human TH mRNA splice variants (hTH1–hTH4) have been identified, differing in their N-terminal regulatory domains while sharing identical catalytic domains. TH catalyzes the conversion of L-tyrosine to L-dopa and plays a key role in adrenergic neuron physiology. Its function is associated with several neuropathogenic diseases, including Parkinson’s disease, schizophrenia, Segawa syndrome, dystonia, and various cardiovascular disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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