Thermo Fisher Scientific Sp1 Polyclonal Antibody
Sp1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, IP 등 다양한 응용에 사용 가능. 액상 형태로 4°C 보관하며, FFPE 조직에서 citrate buffer pH 6.0을 이용한 epitope retrieval 권장. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 1–4 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 700 and 740 of SP1 (UniProt ID: P08047-1, NCBI Node ID: 9606) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rat
Target Information
SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. It binds with high affinity to GC-rich motifs and regulates the expression of numerous genes involved in cell growth, apoptosis, differentiation, and immune responses. SP1 activity is modulated by post-translational modifications such as phosphorylation, sumoylation, proteolytic cleavage, glycosylation, and acetylation. It also binds the PDGFR-alpha G-box promoter and may play a role in modulating the cellular response to DNA damage, chromatin remodeling, and recruitment of SMARCA4/BRG1 on the c-FOS promoter. It is essential for FE65 gene expression regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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