Thermo Fisher Scientific Phospho-INSR (Tyr1334) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:250

-

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Insulin Receptor that contains tyrosine 1334. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:INSR,uniProtId:P06213-1,ncbiNodeId:9606,antigenRange:1334,antigenLength:1382,antigenImageFileName:44-809G_INSR_P06213-1_Rabbit.svg,antigenImageFileNamePDP:44-809G_INSR_P06213-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco`s PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533765

Product Specific Information

This antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Insulin Receptor. The final product is generated by affinity chromatography using a Insulin Receptor-derived peptide that is phosphorylated at tyrosine 1334.

This antibody recognizes human insulin receptor phosphorylated at tyrosine 1334 as well as the human IR Isoform B phosphorylated at tyrosine 1332.

Western blot positive control used was insulin-treated CHO-T cells expressing Human Insulin Receptor.

Target Information

INSR is a receptor tyrosine kinase. Binding of insulin to the extracellular domain of INSR stimulates glucose uptake. INSR and IGF-1 receptors share major structural and functional similarity. The earliest cellular response to insulin stimulation is autophosphorylation of tyrosine in INSR. In humans, the INSR gene is located on chromosome 19. Defects in INSR are the cause of various insulin resistance syndromes and IGF-1R defects may also cause some forms of growth retardation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.