Thermo Fisher Scientific Syk Monoclonal Antibody (4D10.1), Alexa Fluor 488, eBioscience
인간 Syk 단백질을 인식하는 4D10.1 단클론 항체로 Alexa Fluor 488 형광 표지. B세포 및 면역세포 내 신호전달 연구용. Flow Cytometry 분석에 최적화된 프리딜루트 형식. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 53-6696-42
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Syk Monoclonal Antibody (4D10.1), Alexa Fluor 488, eBioscience
Thermo Fisher Scientific Syk Monoclonal Antibody (4D10.1), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (intracellular staining)
- Tested Dilution: 5 µL (0.125 µg)/test
Publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4D10.1 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848443 |
Additional Formats
Product Specific Information
This 4D10.1 monoclonal antibody recognizes human Syk, a 72-kDa member of the Syk/ZAP-70 family of non-receptor protein tyrosine kinases. Syk is highly expressed in B cells, with lower expression in immature thymocytes, mast cells, and platelets. It can also be detected in fibroblasts, epithelial cells, hepatocytes, endothelial cells, and neuronal cells.
Syk plays a key role in signaling cascades downstream of B and T cell antigen receptors and is essential for lymphocyte development. Upon recruitment to ITAMs on antigen receptors, Syk is activated by phosphorylation and subsequently phosphorylates proteins such as phospholipase C gamma and BLNK/SLP-65. Abnormal Syk expression has been linked to tumor cell migration and invasion in several cancers.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-diluted and validated using normal human peripheral blood cells with the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00).
Recommended usage: 5 µL (0.125 µg) per test (100 µL final volume). Cell number per test may vary from 10^5 to 10^8 cells.
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Target Information
Spleen tyrosine kinase (SYK) and ZAP70 are cytoplasmic tyrosine kinases implicated in allergic disorders, autoimmune diseases, and cancer. SYK translocates to the plasma membrane upon BCR or IgE receptor triggering and phosphorylates adaptor proteins, initiating downstream signaling such as calcium mobilization, cytoskeleton remodeling, and gene transcription. SYK interacts with phosphorylated ITAM motifs via its SH2 domains, with Src-family kinases contributing to its activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.