Thermo Fisher Scientific Amplex Red Phosphatidylcholine-Specific Phospholipase C Assay Kit

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형광 기반으로 Phosphatidylcholine-specific Phospholipase C(PC-PLC) 활성을 민감하게 검출하는 키트. 낮은 농도(0.2 mU/mL)까지 측정 가능하며, 시간 경과 측정에 적합. 생물학적 시료의 자동형광 간섭을 최소화하도록 설계됨.

카탈로그번호: A12218
브랜드: Thermo Fisher Scientific
카테고리: Assay Kits
판매단위: pk

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마지막 업데이트 2025. 08. 03. 오전 05:35

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Thermo Fisher Scientific A12218 Amplex Red Phosphatidylcholine-Specific Phospholipase C Assay Kit Each pk판매 단위 pk ·

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680,400원VAT 포함 748,440원

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Thermo Fisher Scientific · Thermo Fisher Scientific Amplex Red Phosphatidylcholine-Specific Phospholipase C Assay Kit

Invitrogen™ Amplex™ Red Phosphatidylcholine-Specific Phospholipase C Assay Kit

The Amplex™ Red Phosphatidylcholine-Specific Phospholipase C Assay Kit provides a sensitive method for detecting phosphatidylcholine-specific phospholipase C (PC-PLC) activity using a fluorescence microplate reader or fluorometer.

Detects phospholipase-C activity levels as low as 0.2 mU/mL
Format allows for multiple time point measurements
Designed for minimal autofluorescence interference

Assay Principle

In this enzyme-coupled assay, PC-PLC activity is monitored indirectly using 10-acetyl-3,7-dihydroxyphenoxazine (Amplex™ Red reagent), a sensitive fluorogenic probe for hydrogen peroxide.

PC-PLC converts phosphatidylcholine (lecithin) to phosphocholine and diacylglycerol.
Alkaline phosphatase hydrolyzes phosphorylcholine to form choline.
Choline is oxidized by choline oxidase to form betaine and hydrogen peroxide.
In the presence of horseradish peroxidase (HRP), hydrogen peroxide reacts with Amplex™ Red reagent to generate the highly fluorescent product resorufin.

Resorufin exhibits absorption and fluorescence emission maxima at approximately 571 nm and 585 nm, respectively, resulting in minimal interference from autofluorescence in most biological samples.

Applications

Use Amplex™ Red Assays for a broad range of investigations, including:

Cell signaling and lipid studies
Neurobiology research
Inflammation and immune function assays
Metabolism studies

Additional related reagents:

Amplex™ UltraRed Reagent (Cat. No. A36006): second-generation reagent with greater sensitivity and brightness
Amplex™ Red/UltraRed Stop Reagent (Cat. No. A33855): enables controlled termination of fluorescence reaction; signal remains stable for at least 3 hours

Custom assay design and packaging options are available.

Specifications

항목	내용
검출 방법	Fluorescence
용도 (애플리케이션)	Phospholipase Assay
사용 장비	Fluorometer, Microplate Reader
제품 유형	Red Phosphatidylcholine-Specific Phospholipase C Assay Kit
염료 유형	Other Label(s) or Dye(s)
형식	Cuvettes, 96-well plate
제품 라인	Amplex™
수량	500 assays
배송 조건	Room Temperature
Unit Size	Each