Thermo Fisher Scientific HLA-DR (MHC II) Monoclonal Antibody (SPM289)

Applications and Tested Dilution

Product Specifications

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and a 27 kDa beta chain (DRB).
The alpha chain gene contains 5 exons: exon 1 encodes the leader peptide, exons 2 and 3 encode the extracellular domains, and exon 4 encodes the transmembrane domain and cytoplasmic tail.
DRA lacks polymorphisms in the peptide-binding region and serves as the sole alpha chain for DRB1, DRB3, DRB4, and DRB5.
The beta chain holds the polymorphisms determining peptide-binding specificities. Hundreds of DRB1 alleles are known, and typing these polymorphisms is standard in bone marrow and kidney transplantation.

HLA-DR is primarily expressed on antigen-presenting cells such as:

• B lymphocytes
• Monocytes
• Macrophages
• Thymic epithelial cells
• Activated T lymphocytes

Three loci—DR, DQ, and DP—encode major expressed products of the human class II region.
Human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and play a critical role in initiating immune responses.

HLA and MHC antibodies are essential in Immunopeptidomics, aiding in the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.