Thermo Fisher Scientific NFYC Polyclonal Antibody
Rabbit polyclonal antibody targeting NFYC (CBF-C) for WB, IHC, ICC/IF applications. Detects endogenous total CBF-C. Supplied as liquid, unconjugated, 1 mg/mL concentration. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human (unreviewed) hCBF-C (Accession Q14497), corresponding to amino acid residues T10–R25. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817655 |
Product Specific Information
Antibody detects endogenous levels of total CBF-C.
Target Information
The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is composed of three subunits: NF-YA, NF-YB, and NF-YC. All three components are required for DNA binding.
Each NF-Y subunit contains a conserved segment necessary for NF-Y–DNA complex formation, homologous to the histone-fold motif of eukaryotic histones. The DNA-binding domains of NF-YB and NF-YC interact through a histone-fold “handshake” motif similar to histone proteins H2B and H2A.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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