Thermo Fisher Scientific MEKK1 Polyclonal Antibody
Rabbit polyclonal antibody against human MEKK1, validated for Western blot and immunoprecipitation. Recognizes conserved MAPK pathway kinase with cross-reactivity to mouse and rat. Supplied as liquid at 1 mg/mL, stored at 4 °C. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 5–10 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 50 and 100 of human MAP/ERK kinase kinase 1 (MEKK1, UniProt ID: Q13233‑1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4 °C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat
Target Information
Mitogen‑activated protein kinase (MAPK) signaling cascades include MAPK (ERK), MAPK kinase (MKK/MEK), and MAPK kinase kinase (MAPKKK/MEKK).
MEKK1 phosphorylates and activates MAPKK 1 and MAPKK 2 (MEK1/MEK2), leading to phosphorylation of MAP kinases and activation of the JNK cascade.
The protein contains a putative RING‑type zinc finger and a SWIM‑type zinc finger.
These kinases are highly conserved across species including yeast, Drosophila, and mammals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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