Thermo Fisher Scientific PFKFB2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

The recommended shelf life for this product is 1 year from the date of receipt.

Target Information

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the γ phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation.

With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified into 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases, including:

• Cyclic nucleotide-regulated protein kinases (PKA & PKG)
• Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC)
• Related to PKA and PKC (RAC/Akt) protein kinase family
• Kinases that phosphorylate G protein-coupled receptors (ARK)
• Kinases that phosphorylate ribosomal protein S6 (RSK)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.