Thermo Fisher Scientific PHIP Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human PHIP. Recombinant protein control fragment (Product #RP-92477). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PHIP,uniProtId:Q8WWQ0-1,ncbiNodeId:9606,antigenRange:1615-1739,antigenLength:1821,antigenImageFileName:PA5-53938_PHIP_Q8WWQ0-1_Rabbit.svg,antigenImageFileNamePDP:PA5-53938_PHIP_Q8WWQ0-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2645504

Product Specific Information

Immunogen sequence: NALVPGTIQV NGHGGQPSKL VKRGPGRKPK VEVNTNSGEI IHKKRGRKPK KLQYAKPEDL EQNNVHPIRD EVLPSSTCNF LSETNNVKED LLQKKNRGGR KPKRKMKTQK LDADLLVPAS VKVLR

Highest antigen sequence identity to the following orthologs: Mouse - 82%, Rat - 79%.

Target Information

PHIP (pleckstrin homology domain interacting protein), also known as ndrp or WDR11, is a 1,821 amino acid protein that contains eight N-terminal WD40 repeats and two bromodomains. It is expressed in skeletal muscle (localizing to the cytosol and nucleus) and primary beta cells (localizing to the nucleus) and acts as a transcriptional activator. PHIP is known to interact with various members of the insulin receptor substrate (IRS) family. The IRS family of proteins mediate insulin receptor signaling and play an important role in insulin-producing beta cell proliferation and survival. PHIP specifically associates with the PH domain of IRS-1 and may function to link IRS-1 to insulin receptors, indicating a vital role of PHIP in the regulation of insulin signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.