Thermo Fisher Scientific NMT2 Monoclonal Antibody (OTI2E1), TrueMAB

Applications and Tested Dilution

Product Specifications

Target Information

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signaling factors.
The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus, while others are degraded via the vacuolar pathway, which includes endosomes, lysosomes, and the endoplasmic reticulum.
The 26S proteasome is an ATP-dependent, multisubunit (approximately 31), barrel-shaped molecular machine with an apparent molecular weight of approximately 2.5 MDa. It consists of a 20S proteolytic core complex crowned at one or both ends by 19S regulatory subunit complexes.
The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit.
An enzymatic cascade attaches multiple ubiquitin molecules to lysine residues of proteins targeted for degradation.
Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway, including cystic fibrosis, Angelman’s syndrome, and Liddle syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.